Composite

Part:BBa_K3320014

Designed by: Joseana Mendes Comodaro   Group: iGEM19_Amazonas-Brazil   (2019-10-20)


lldOJ23117lldO + with PhlF repressor

The first part of the lactate repressor unit from our AND Gate.


Usage and Biology

The J23117 promoter is intercalated between two operators, which regulates the expression of the phlF repressor, this protein is capable of repressing the phlF promoter from the BBa_K3320015.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None